Publications
Publications
Browse IGI publications in peer-reviewed journals, organized by year and sortable by research program. Click the Altmetric badge for details on social media engagement and press coverage related to each publication.
Functional protein mining with conformal guarantees
Boger RS, Chithrananda S, Angelopoulos AN, Yoon PH, Jordan MI, and Doudna JA. Nature Communications
Mechanism-guided engineering of a minimal biological particle for genome editing
Ngo W, Peukes J, Baldwin A, Xue ZW, Hwang S, Stickels RR, Lin Z, Satpathy AT, Wells JA, Schekman R, Nogales E, and Doudna JA. PNAS
CRISPR-Cas12a bends DNA to destabilize base pairs during target interrogation
Soczek KM, Cofsky JC, Tuck OT, Shi H, and Doudna JA. Nucleic Acids Research
RNA elements required for the high efficiency of West Nile virus-induced ribosomal frameshifting
Aleksashin NA, Langeberg CJ, Shelke RR, Yin T, Cate JHD. Nucleic Acids Research
Diverse microbiome functions, limited temporal variation and substantial genomic conservation within sedimentary and granite rock deep underground research laboratories
Amano Y, Sachdeva R, Gittins D, Anantharaman K, Lei S, Valentin-Alvarado LE, Diamond S, Beppu H, Iwatsuki T, Mochizuki A, Miyakawa K, Ishii E, Murakami H, Jaffe AL, Castelle C, Lavy A, Suzuki Y, and Banfield JF. Environmental Microbiome
Central control of dynamic gene circuits governs T cell rest and activation
Arce MM, Umhoefer JM, Arang N, Kasinathan S, Freimer JW, Steinhart Z, Shen H, Pham MTN, Ota M, Wadhera A, Dajani R, Dorovskyi D, Chen YY, Liu Q, Zhou Y, Swaney DL, Obernier K, Shy BR, Carnevale J, Satpathy AT, Krogan NJ, Pritchard JK, and Marson A. Nature
A technical approach to global plant genome editing regulation
Groover E, Njuguna E, Bansal KC, Muia A, Kwehangana M, Simuntala C, Mills RL, Kwakye E, Rocha P, Amedu J, Morillo E, Anthonysamy MA, Khaldun ABM, Chimpepo L, Akoudjin M, Senanayake DMJB, Wangmo D, Atnafu D, Eusebio GP, Kongsawat C, and Kliegman M. Nature Biotechnology
MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration
Zdouc MM, Blin K, Louwen NLL … Creamer KE, Kust A … et. al. Nucleic Acids Research
RNA language models predict mutations that improve RNA function
Shuglina Y, Trinidad MI, Langeberg CJ, Nisonoff H, Chithrananda S, Skopintsev P, Nissley AJ, Patel J, Boger RS, Shi H, Yoon PH, Doherty EE, Pande T, Iyer AM, Doudna JA, and Cate JHD. Nature Communications
Cyanobacteria from marine oxygen-deficient zones encode both form I and form II Rubiscos
Jaffe AL, Harrison K, Wang RZ, Taylor-Kearney LJ, Jain N, Prywes N, Shih PM, Young J, Rocap G, and Dekas AE. PNAS
SARS-CoV-2 evolution balances conflicting roles of N protein phosphorylation
Syed AM, Ciling A, Chen IP, Carlson CR, Adly AN, Martin HS, Taha TY, Khalid MM, Price N, Bouhaddou M, Ummadi MR, Moen JM, Krogan NJ, Morgan DO, Ott M, and Doudna JA. PLoS Pathogens
A self-eliminating allelic-drive reverses insecticide resistance in Drosophila leaving no transgene in the population
Auradkar A, Corder RM, Marshall JM, and Bier E. Nature Communications
Towards targeted engineering of promoters via deletion of repressive cis-regulatory elements
Wang FZ and Niyogi KK. New Phytologist
Synergistic induction of fertilization-independent embryogenesis in rice egg cells by paternal-genome-expressed transcription factors
Ren H, Shankle K, Cho MJ, Tjahjadi M, Khanday I, and Sundaresan V. Nature Plants
Binary vector copy number engineering improves Agrobacterium-mediated transformation
Szarzanowicz MJ, Waldburger LM, Busche M, Geiselman GM, Kirkpatrick LD, Kehl AJ, Tahmin C, Kuo RC, McCauley J, Pannu H, Cui R, Liu S, Hillson NJ, Brunkard JO, Keasling JD, Gladden JM, Thompson MG, and Shih PM. Nature Biotechnology
Widespread Gene Editing in the Brain via In Utero Delivery of mRNA Using Acid-Degradable Lipid Nanoparticles
Szarzanowicz MJ, Waldburger LM, Busche M, Geiselman GM, Kirkpatrick LD, Kehl AJ, Tahmin C, Kuo RC, McCauley J, Pannu H, Cui R, Liu S, Hillson NJ, Brunkard JO, Keasling JD, Gladden JM, Thompson MG, and Shih PM. Nature Biotechnology
Complete genomes of Asgard archaea reveal diverse integrated and mobile genetic elements
Valentin-Alvarado LE, Shi L-D, Appler KE, Crits-Christoph A, De Anda V, Adler BA, Cui ML, Ly L, Leão P, Roberts RJ, Sachdeva R, Baker BJ, Savage DF, and Banfield JF. Genome Research
Give Cas a Chance: An Actionable Path to a Platform for CRISPR Cures
Urnov FD. The CRISPR Journal
Engineering quantitative stomatal trait variation and local adaptation potential by cis-regulatory editing
Karavolias NG, Patel-Tupper D, Gallegos Cruz A, Litvak L, Lieberman SE, Tjahjadi M, Niyogi KK, Cho MJ, and Staskawicz BJ. Plant Biotechnology Journal
Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR–Cas9 ribonucleoprotein
Chen K, Han H, Zhao S, Xu B, Yin B, Lawanprasert A, Trinidad M, Burgstone BW, Murthy N, Doudna JA. Nature Biotechnology
Genome Editing Therapy for the Blood: Ex Vivo Success and In Vivo Prospects
George CA, Sahu SU, de Oñate L, Solano de Freitas Souza B, and Wilson RC. The CRISPR Journal
Ethylene oxide graft copolymers reduce the immunogenicity of lipid nanoparticles
Qi Y, Han H, Liu A, Zhao S, Lawanprasert A, Nielsen JE, Choudhary H, Liang D, Barron AE, and Murthy N. RSC Advances
Modern microbiology: Embracing complexity through integration across scales
Eren AM and Banfield JF. Cell
Acid-degradable lipid nanoparticles enhance the delivery of mRNA
Zhao S, Gao K, Han H, Stenzel M, Yin B, Song H, Lawanprasert A, Nielsen JE, Sharma R, Arogundade OH, Pimcharoen S, Chen Y-J, Paul A, Tuma J, Collins MG, Wyle Y, Cranick MG, Burgstone BW, Perez BS, Barron AE, Smith AM, Lee HY, Wang A, and Murthy N. Nature Nanotechnology
Animal and bacterial viruses share conserved mechanisms of immune evasion
Hobbs SJ, Nomburg J, Doudna JA, and Kranzusch PJ. Cell
Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly
Turnšek JB, Oltrogge LM, and Savage DF. Journal of Biological Chemistry
Evolution and origins of rubisco
Taylor-Kearney LJ, Wang RZ, and Shih PM. Current Biology
Desiccated cyanobacteria serve as efficient plasmid DNA carriers in space flight
Kakouridis A, Diamond S, Eng T, Mills HJ, Gámez Holzhaus O, Summers ML, Garcia-Pichel F, and Mukhopadhyay A. ACS Synthetic Biology
Activation of the helper NRC4 immune receptor forms a hexameric resistosome
Liu F, Yang Z, Wang C, You Z, Martin R, Qiao W, Huang J, Jacob P, Carette JE, Luan S, Nogales E, and Staskawicz B. Cell
Asgard archaea modulate potential methanogenesis substrates in wetland soil
Valentin-Alvarado LE, Appler KE, De Anda V, Schoelmerich MC, West-Roberts J, Kivenson V, Crits-Christoph A, Ly L, Sachdeva R, Greening C, Savage DF, Baker BJ, and Banfield JF. Nature Communications
A roadmap for affordable genetic medicines
Kliegman M, Zaghlula M, Abrahamson S, Esensten JH, Wilson R., Urnov FD, and Doudna JA. Nature
Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly
Turnsek JB, Oltrogge LM, and Savage DF. Journal of Biological Chemistry
A Translation-Independent Directed Evolution Strategy to Engineer Aminoacyl-tRNA Synthetases
Soni C, Prywes N, Hall M, Nair MA, Savage DF, Schepartz A, and Chatterjee A. ACS Central Science
Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
Schoelmerich MC, Ly L, West-Roberts J, Shi LD, Shen C, Malvankar NS, Taib N, Gribaldo S, Woodcroft BJ, Schadt CW, Al-Shayeb B, Dai X, Mozsary C, Hickey S, He C, Beaulaurier J, Juul S, Sachdeva R, and Banfield JF. Nature Communications
Methanotrophic Methanoperedens archaea host diverse and interacting extrachromosomal elements
Shi LD, West-Roberts J, Schoelmerich MC, Penev PI, Chen L, Amano Y, Lei S, Sachdeva R, and Banfield JF. Nature Microbiology
Eliminating malaria vectors with precision-guided sterile males
Apte RA, Smidler AL, Pai JJ, Chow ML, Chen S, Mondal A, Sánchez C HM, Antoshechkin I, Marshall JM, Akbari OS. PNAS
Enhancing pediatric access to cell and gene therapies
Mackall CL, Bollard CM, Goodman N, Carr C, Gardner R, Rouce R, Sotillo E, Stoner R, Urnov FD, Wayne AS, Park J, and Kohn DB. Nature Medicine
Engineered plants provide a photosynthetic platform for the production of diverse human milk oligosaccharides
Barnum CR, Paviani B, Couture G, Masarweh C, Chen Y, Huang Y-P, Markel K, Mills DA, Lebrilla CB, Barile D, Yang M, and Shih PM. Nature Food
Multiplexed CRISPR-Cas9 mutagenesis of rice PSBS1 noncoding sequences for transgene-free overexpression
Patel-Tupper D, Kelikian A, Leipertz A, Maryn N, Tjahjadi M, Karavolias NG, Cho M-J, and Niyogi KK. Science Advances
Systematic decoding of cis gene regulation defines context-dependent control of the multi-gene costimulatory receptor locus in human T cells
Mowery CT, Freimer JW, Chen Z, Casaní-Galdón S, Umhoefer JM, Arce MM, Gjoni K, Daniel B, Sandor K, Gowen BG, Nguyen V, Simeonov DR, Garrido CM, Curie GL, Schmidt R, Steinhart Z, Satpathy AT, Pollard KS, Corn JE, Bernstein BE, Ye CJ, and Marson A. Nature Genetics
The gut–airway microbiome axis in health and respiratory diseases
Özçam M and Lynch SV. Nature Reviews Microbiology
Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, and Doudna JA. Cell
We need to act now to ensure global food security, and reduce agricultural greenhouse gas emissions
Ringeisen BR, de Azevedo Souza C, Njuguna EW, and Ronald PC. Bulletin of the Atomic Scientists
An essential and highly selective protein import pathway encoded by nucleus-forming phage
Morgan CJ, Enustun E, Armbruster EG, Birkholz EA, Prichard A, Forman T, Aindow A, Wannasrichan W, Peters S, Inlow K, Shepherd IL, Razavilar A, Chaikeeratisak V, Adler BA, Cress BF, Doudna JA, Pogliano K, Villa E, Corbett KD, and Pogliano J. PNAS
Perspectives on Genetically Engineered Microorganisms and Their Regulation in the United States
Shams A, Fischer A, Bodnar A, and Kliegman M. ACS Synthetic Biology
The plant immune system: From discovery to deployment
Jones JDG, Staskawicz BJ, and Dangl JL. Cell
Reversal of C9orf72 mutation-induced transcriptional dysregulation and pathology in cultured human neurons by allele-specific excision
Sachdev A, Gill K, Sckaff M, Birk AM, Aladesuyi Arogundade O, Brown KA, Chouhan RS, Issagholian-Lewin PO, Patel E, Watry HL, Bernardi MT, Keough KC, Tsai YC, Smith AST, Conklin BR, and Clelland CD. PNAS
Assembly of SARS-CoV-2 nucleocapsid protein with nucleic acid
Zhao H, Syed AM, Khalid MM, Nguyen A, Ciling A, Wu D, Yau WM, Srinivasan S, Esposito D, Doudna JA, Piszczek G, Ott M, and Schuck P. Nucleic Acids Research
Improving prime editing with an endogenous small RNA-binding protein
Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, & Adamson B. Nature
A phage nucleus-associated RNA-binding protein is required for jumbo phage infection
Enustun E, Armbruster EG, Lee J, Zhang S, Yee BA, Malukhina K, Gu Y, Deep A, Naritomi JT, Liang Q, Aigner S, Adler BA, Cress BF, Doudna JA, Chaikeeratisak V, Cleveland DW, Ghassemian M, Bintu B, Yeo GW, Pogliano J, Corbett KD. Nucleic Acids Research
CRISPR-based split luciferase as a biosensor for unique DNA sequences in situ
Heath NG and Segal DJ. Methods in Molecular Biology
Targeted nonviral delivery of genome editors in vivo
Tsuchida CA, Wasko KM, Hamilton JR, and Doudna JA. PNAS
Engineering Brassica crops to optimize delivery of bioactive products postcooking
Barnum CR, Cho M-J, Markel K, and Shih PM. ACS Synthetic Biology
Engineering self-deliverable ribonucleoproteins for genome editing in the brain
Chen K, Stahl EC, Kang MH, Xu B, Allen R, Trinidad M, and Doudna JA. Nature Communications
Opportunities and challenges for innovative and equitable healthcare
Ecker DJ, Aiello CD, Arron JR, Bennett CF, Bernard A, Breakefield XO, Broderick TJ, Callier SL, Canton B, Chen JS, Fishburn CS, Garrett B, Hecht SM, Janowitz T, Kliegman M, Krainer A, Louis CU, Lowe C, Sehgal A, Tozan Y, Tracey KJ, Urnov F, Wattendorf D, Williams TW, Zhao X, and Hayden MR. Nature Reviews Drug Discovery
Protein design using structure-based residue preferences
Ding D, Shaw AY, Sinai S, Rollins N, Prywes N, Savage DF, Laub MT, and Marks DS. Nature Communications
COBRA improves the completeness and contiguity of viral genomes assembled from metagenomes
Chen LX and Banfield JF. Nature Microbiology
Candidate Phyla Radiation bacteria
Jaffe AL and Banfield JF. Current Biology
Digital telomere measurement by long-read sequencing distinguishes healthy aging from disease
Sanchez SE, Gu Y, Wang Y, Golla A, Martin A, Shomali W, Hockemeyer D, Savage SA, and Artandi SE. Nature Communications
In vivo human T cell engineering with enveloped delivery vehicles
Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, Trinidad M, Ngo W, and Doudna JA. Nature Biotechnology
Targeted Protein Degradation through Recruitment of the CUL4 Complex Adaptor Protein DDB1
Meyers M, Cismoski S, Panidapu A, Chie-Leon B, Nomura DK. Targeted Protein Degradation through Recruitment of the CUL4 Complex Adaptor Protein DDB1. ACS Chemical Biology
Infant gut DNA bacteriophage strain persistence during the first 3 years of life
Lou CY, Chen L, Borges AL, West-Roberts J, Firek BA, Morowitz MJ, and Banfield JF.
Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages
Piya D, Nolan N, Moore ML, Ramirez Hernandez LA, Cress BF, Young R, Arkin AP, and Mutalik VK. PLOS Biology
Host translation machinery is not a barrier to phages that interact with both CPR and non-CPR bacteria
Liu J, Jaffe AL, Chen L, Bor B, and Banfield JF. mBio
Infant microbiome cultivation and metagenomic analysis reveal Bifidobacterium 2′-fucosyllactose utilization can be facilitated by coexisting species
Lou YC, Rubin BE, Schoelmerich MC, DiMarco KS, Borges AL, Rovinsky R, Song L, Doudna JA, and Banfield JF. Nature Communications
Eukaryotic RNA-guided endonucleases evolved from a unique clade of bacterial enzymes
Yoon PH, Skopintsev P, Shi H, Chen L, Adler BA, Al-Shimary M, Craig RJ, Loi KJ, DeTurk EC, Li Z, Amerasekera J, Trinidad M, Nisonoff H, Chen K, Lahiri A, Boger R, Jacobsen S, Banfield JF, an Doudna JA. Nucleic Acids Research
Deep-branching evolutionary intermediates reveal structural origins of form I rubisco
Liu AK, Kaeser B, Chen L, West-Roberts J, Taylor-Kearney LJ, Lavy A, Günzing D, Li WJ, Hammel M, Nogales E, Banfield JF, and Shih PM. Current Biology
Towards affordable CRISPR genomic therapies: a task force convened by the Innovative Genomics Institute
Witkowsky L, Norstad M, Glynn AR, and Kliegman M. Gene Therapy
Tracing cancer evolution and heterogeneity using Hi-C
Erdmann-Pham DD, Batra SS, Turkalo TK, Durbin J, Blanchette M, Yeh I, Shain H, Bastian BC, Song YS, Rokhsar DS, and Hockemeyer D. Nature Communications
Targeting the non-coding genome and temozolomide signature enables CRISPR-mediated glioma oncolysis
Tan IL, Perez AR, Lew RJ, Sun X, Baldwin A, Zhu YK, Shah MM, Berger MS, Doudna JA, and Fellmann C. Cell Reports
CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes
Adler BA, Trinidad MI, Bellieny-Rabelo D, Zhang E, Karp HM, Skopintsev P, Thornton BW, Weissman RF, Yoon PH, Chen L, Hessler T, Eggers AR, Colognori D, Boger R, Doherty EE, Tsuchida CA, Tran RV, Hofman L, Shi H, Wasko KM, Zhou Z, Xia C, Al-Shimary MJ, Patel JR, Thomas VCJX, Pattali R, Kan MJ, Vardapetyan A, Yang A, Lahiri A, Maxwell MF, Murdock AG, Ramit GC, Henderson HR, Calvert RW, Bamert RS, Knott GJ, Lapinaite A, Pausch P, Cofsky JC, Sontheimer EJ, Wiedenheft B, Fineran PC, Brouns SJJ, Sashital DG, Thomas BC, Brown CT, Goltsman DSA, Barrangou R, Siksnys V, Banfield JF, Savage DF, and Doudna JA. Nucleic Acids Research
Implementation of California COVIDNet – a multi-sector collaboration for statewide SARS-CoV-2 genomic surveillance
Wadford DA, Baumrind N, Baylis EF…Corbett-Detig RB, DeRisi J, Jacobson K and the COVIDNet Consortium. Frontiers in Public Health
Previous exposure to Spike-providing parental strains confers neutralizing immunity to XBB lineage and other SARS-CoV-2 recombinants in the context of vaccination
Assembly of SARS-CoV-2 ribonucleosomes by truncated N* variant of the nucleocapsid protein
Adly AN, Bi M, Carlson CR, Syed AM, Ciling A, Doudna JA, Cheng Y, and Morgan DO. Journal of Biological Chemistry
The NLR immune receptor ADR1 and lipase-like proteins EDS1 and PAD4 mediate stomatal immunity in Nicotiana benthamiana and Arabidopsis
Wang H, Song S, Gao S, Yu Q, Zhang H, Cui X, Fan J, Xin X, Liu Y, Staskawicz B, and Qi T. Plant Cell
Single-nuclei characterization of pervasive transcriptional signatures across organs in response to COVID-19
COVID Tissue Atlas Consortium, Granados AA, Bucher S, Song H, Agrawal A, Chen AT, Peng T, Neff N, Pisco AO, Huang F, Wang B. eLife
Nuclear transport receptor KA120 regulates molecular condensation of MAC3 to coordinate plant immune activation
Jia M, Chen X, Shi X, Fang Y, and Gu Y. Cell Host & Microbe
A highly efficient human cell-free translation system
Aleksashin NA, Chang ST, and Cate JHD. RNA
Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells
Tsuchida CA, Brandes N, Bueno R, Trinidad M, Mazumder T, Yu B, Hwang B, Chang C, Liu J, Sun Y, Hopkins CR, Parker KR, Qi Y, Hofman L, Satpathy AT, Stadtmauer EA, Cate JHD, Eyquem J, Fraietta JA, June CH, Chang HY, Ye CJ, and Doudna JA
Covalent Targeting of Glutamate Cysteine Ligase to Inhibit Glutathione Synthesis
Zhang LH, Tang M, Tao X, Shao Q, Thomas V, Shimizu S, Kasano M, Ishikawa Y, Inukai T, and Nomura DK. ChemBioChem
Rhizosphere and detritusphere habitats modulate expression of soil N-cycling genes during plant development
Sieradzki ET, Nuccio EE, Pett-Ridge J, and Firestone MK. Rhizosphere and detritusphere habitats modulate expression of soil N-cycling genes during plant development. mSystems
SARS-CoV-2 variants evolve convergent strategies to remodel the host response
Stevenson E, Rojc A, Ragazzini R, Whelan MVX, Furnon W, De Lorenzo G, Cowton V, Syed AM, Ciling A, Deutsch N, Pirak D, Dowgier G, Mesner D, Turner JL, McGovern BL, Rodriguez ML, Leiva-Rebollo R, Dunham AS, Zhong X, Eckhardt M, Fossati A, Liotta NF, Kehrer T, Cupic A, Rutkowska M, Mena I, Aslam S, Hoffert A, Foussard H, Olwal CO, Huang W, Zwaka T, Pham J, Lyons M, Donohue L, Griffin A, Nugent R, Holden K, Deans R, Aviles P, Lopez-Martin JA, Jimeno JM, Obernier K, Fabius JM, Soucheray M, Hüttenhain R, Jungreis I, Kellis M, Echeverria I, Verba K, Bonfanti P, Beltrao P, Sharan R, Doudna JA, Martinez-Sobrido L, Patel AH, Palmarini M, Miorin L, White K, Swaney DL, Garcia-Sastre A, Jolly C, Zuliani-Alvarez L, Towers GJ, Krogan NJ. Cell
A subset of viruses thrives following microbial resuscitation during rewetting of a seasonally dry California grassland soil
Nicolas AM, Sieradzki ET, Pett-Ridge J, Banfield JF, Taga ME, Firestone MK and Blazewicz SJ.
Lipid Nanoparticles Deliver mRNA to the Brain after an Intracerebral Injection
Tuma J, Chen YJ, Collins MG, Paul A, Li J, Han H, Sharma R, Murthy N, Lee HY. Biochemistry
Manipulating the Destiny of Wild Populations Using CRISPR
Raban R, Marshall JM, Hay BA, and Akbari OS.
Modular pooled discovery of synthetic knockin sequences to program durable cell therapies
Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY…Eyquem J, Ye CJ, Carnevale J, Satpathy AT, Shifrut E, Roth TL, and Marson A. Cell
CRISPR screens decode cancer cell pathways that trigger γδ T cell detection
Mamedov MR, Vedova S, Freimer JW, Sahu AD, Ramesh A, Arce MM, Meringa AD, Ota M, Chen PA, Hanspers K, Nguyen VQ, Takeshima KA, Rios AC, Pritchard JK, Kuball J, Sebestyen Z, Adams EJ, Marson A. Nature
Enhancing sustainable development through plant genetics
Ronald PC. Nature Reviews Genetics
A Primer on Gene Editing: What Does It Mean for Pathologists?
Cushman-Vokoun A, Schmidt RJ, Hiemenz MC, Fung M, Zhang BM, Bradshaw G, Gandhi M, Yao J, Yohe S, Beckman A, Grody WW, and Giannikopoulos P. Archives of Pathology & Laboratory Medicine
Efficient parthenogenesis via egg cell expression of maize BABY BOOM 1: a step toward synthetic apomixis
Skinner DJ, Mallari MD, Zafar K, Cho MJ, Sundaresan V. Plant Physiology
Vitamin interdependencies predicted by metagenomics-informed network analyses and validated in microbial community microcosms
Hessler T, Huddy RJ, Sachdeva R, Lei S, Harrison STL, Diamond S, and Banfield JF
Functional annotation of variants of the BRCA2 gene via locally haploid human pluripotent stem cells
Li H, Bartke R, Zhao L, Verma Y, Horacek A, Ben-Natan AR, Pangilinan GR, Krishnappa N, Nielsen R, and Hockemeyer D. Nature Biomedical Engineering
Genome editing in the mouse brain with minimally immunogenic Cas9 RNPs
Stahl EC, Sabo JK, Kang MH, Allen R, Applegate E, Kim SE, Kwon Y, Seth A, Lemus N, Salinas-Rios V, Soczek KM, Trinidad M, Vo LT, Jeans C, Wozniak A, Morris T, Kimberlin A, Foti T, Savage DF, and Doudna JA. Molecular Therapy
Genome expansion by a CRISPR trimmer-integrase
Wang JY, Tuck OT, Skopintsev P, Soczek KM, Li G, Al-Shayeb B, Zhou J, and Doudna JA. Nature
Atomistic simulations of the Escherichia coli ribosome provide selection criteria for translationally active substrates
Watson ZL, Knudson IJ, Ward FR, Miller SJ, Cate JHD, Schepartz A, and Abramyan AM. Nature Chemistry
Aminobenzoic acid derivatives obstruct induced fit in the catalytic center of the ribosome
Majumdar C, Walker JA, Francis MB, Schepartz A, and Cate JHD. ACS Central Science
Three-dimensional genome rewiring in loci with human accelerated regions
Keough KC, Whalen S, Inoue F, Przytycki PF, Fair T, Deng C, Steyert M, Ryu H, Lindblad-Toh K, Karlsson E, Zoonomia Consortium, Nowakowski T, Ahituv N, Pollen AA, and Pollard KS. Science
Rubisco Function, Evolution, and Engineering
Prywes N, Phillips NR, Tuck OT, Valentin-Alvarado LE, Savage DF. Annual Reviews of Biochemistry
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes
Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain C, Li Z, Marson A, Eyquem J, and Wilson RC. Nature Biomedical Engineering
Rapid assembly of SARS-CoV-2 genomes reveals attenuation of the Omicron BA.1 variant through NSP
Taha TY, Chen IP, Hayashi JM, Tabata T, Walcott K, Kimmerly GR, Syed AM, Ciling A, Suryawanshi RK, Martin HS, Bach BH, Tsou CL, Montano M, Khalid MM, Sreekumar BK, Kumar GR, Wyman S, Doudna JA, Ott M. Nature Communications
Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerance
Karavolias NG, Patel-Tupper D, Seong K, Tjahjadi M, Gueorguieva G, Tanaka J, Cruz AG, Lieberman S, Litvak L, Dahlbeck D, Cho MJ, Niyogi KN, and Staskawicz BJ. Plant Physiology
Genetic architecture of human brain evolution
Fair T and Pollen AA. Current Opinion in Neurobiology
The protein corona from nanomedicine to environmental science
Mahmoudi M, Landry MP, Moore A, and Coreas R. Nature Reviews Materials
CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen production
Gomez MA, Berkoff KC, Gill BK, Iavarone AT, Lieberman SE, Ma JM, Schultink A, Karavolias NG, Wyman SK, Chauhan RD, Taylor NJ, Staskawicz BJ, Cho MJ, Rokhsar DS, and Lyons BJ. Frontiers in Plant Science
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data
Austin GI, Park H, Meydan Y, Seeram D, Sezin T, Lou YC, Firek BA, Morowitz MJ, Banfield JF, Christiano AM, Pe’er I, Uhlemann A-C, Shenhav L, and Korem T. Nature Biotechnology
A single-amino acid substitution in the adaptor LAT accelerates TCR proofreading kinetics and alters T-cell selection, maintenance and function
Lo WL, Kuhlmann M, Rizzuto G, Ekiz HA, Kolawole EM, Revelo MP, Andargachew R, Li Z, Tsai YL, Marson A, Evavold BD, Zehn D, Weiss A. Nature Immunology
Using strain-resolved analysis to identify contamination in metagenomics data
Lou YC, Hoff J, Olm MR, West-Roberts J, Diamond S, Firek BA, Morowitz MJ, and Banfield JF. Microbiome
To TnpB or not TnpB? Cas12 is the answer
Yoon PH, Adler BA, and Doudna JA. Nature Chemical Biology
Human-specific genetics: new tools to explore the molecular and cellular basis of human evolution
Pollen AA, Kilik U, Lowe CB, and Camp JG. Nature Reviews Genetics
Somatic embryo initiation by rice BABY BOOM1 involves activation of zygote-expressed auxin biosynthesis genes
Khanday I, Santos-Medellín C, and Sundaresan V. New Phytologist
CRISPR-Csm for eukaryotic RNA knockdown and imaging without toxicity
Colognori D and Doudna JA. Nature Biotechnology (Research briefing on “Precise transcript targeting by CRISPR-Csm complexes,” Nature Biotechnology)
Precise transcript targeting by CRISPR-Csm complexes
Colognori D, Trinidad M, and Doudna JA. Nature Biotechnology
CRISPR technology: A decade of genome editing is only the beginning
Wang YA and Doudna JA. Science
Genetic conversion of a split-drive into a full-drive element
Terradas G, Bennett J, Li Z, Marshall JM, and Bier E. Nature Communications
Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Triticum aestivum L.)
Poddar S, Tanaka J, Running KLD, Kariyawasam GK, Faris JD, Friesen TL, Cho M-J, Cate JHD, and Staskawicz B. Frontiers in Plant Science
Prediction of effector protein structures from fungal phytopathogens enables evolutionary analyses
Seong K and Krasileva KV. Nature Microbiology
High-frequency synthetic apomixis in hybrid rice
Vernet A, Meynard D…Khanday I, Mercier R, Sundaresan V, and Guiderdoni E. Nature Communications
Exploring the value of a global gene drive project registry
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Lingeman E, Jeans C, and Corn JE. Current Protocols in Molecular Biology
Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair
Lee K, Conboy M, Park HM, Jiang F, Kim HJ, Dewitt MA, Mackley VA, Chang K, Rao A, Skinner C, Shobha T, Mehdipour M, Liu H, Huang WC, Lan F, Bray NL, Li S, Corn JE, Kataoka K, Doudna JA, Conboy I, Murthy N. Nature Biomedical Engineering
Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements.
Mumbach MR, Satpathy AT, Boyle EA, Dai C, Gowen BG, Cho SW, Nguyen ML, Rubin AJ, Granja JM, Kazane KR, Wei Y, Nguyen T, Greenside PG, Corces MR, Tycko J, Simeonov DR, Suliman N, Li R, Xu J, Flynn RA, Kundaje A, Khavari PA, Marson A, Corn JE, Quertermous T, Greenleaf WJ, and Chang HY. Nature Genetics. Preprint (free to read).
Discovery of stimulation-responsive immune enhancers with CRISPR activation.
Simeonov DR, Gowen BG, Boontanrart M, Roth TL, Gagnon JD, Mumbach MR, Satpathy AT, Lee Y, Bray NL, Chan AY, Lituiev DS, Nguyen ML, Gate RE, Subramaniam M, Li Z, Woo JM, Mitros T, Ray GJ, Curie GL, Naddaf N, Chu JS, Ma H, Boyer E, Van Gool F, Huang H, Liu R, Tobin VR, Schumann K, Daly MJ, Farh KK, Ansel KM, Ye CJ, Greenleaf WJ, Anderson MS, Bluestone JA, Chang HY, Corn JE, and Marson A. Nature. Preprint (free to read).
Disabling Cas9 by an anti-CRISPR DNA mimic.
Shin J, Jiang F, Liu J, Bray NL, Rauch BJ, Baik SH, Nogales E, Corn JE, and Doudna JA. Science Advances
Targeted gene knock-in by homology-directed genome editing using Cas9 ribonucleoprotein and AAV donor delivery
Gaj T, Staahl BT, Rodrigues GMC, Limsirichai P, Ekman FK, Doudna JA, Schaffer DV. Nucleic Acids Research
Between-region genetic divergence reflects the mode and tempo of tumor evolution.
Sun R, Hu Z, Sottoriva A, Graham TA, Harpak A, Ma Z, Fischer JM, Shibata D, and Curtis C. Nature Genetics
Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering.
Lee K, Mackley VA, Rao A, Chong AT, Dewitt MA, Corn JE, and Murthy N. eLife
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA. Nature Biotechnology
Genome editing via delivery of Cas9 ribonucleoprotein.
DeWitt M, Carroll D, and Corn JE. Methods
A population genetics perspective on the determinants of intra-tumor heterogeneity.
Hu Z, Sun R, and Curtis C. Biochimica et Biophysica Acta
A multiplexed single-cell CRISPR screening platform enables systematic dissection of the unfolded protein response.
Adamson B, Norman TM, Jost M, Cho MY, Nuñez JK, Chen Y, Villalta JE, Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit A, Parnas O, Regev A, and Weissman JS. Cell
Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells.
DeWitt MA, Magis W, Bray NL, Wang T, Berman JR, Urbinati F, Heo S, Mitros T, Muñoz DP, Boffelli D, Kohn DB, Walters MC, Carroll D, Martin DK, and Corn JE. Science Translational Medicine. Preprint (free to read).
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.
Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, and Weissman JS. eLife
Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes.
Richardson CD, Ray GJ, Bray NL, and Corn JE. Nature Communications
Inferring tumor phylogenies from multi-region sequencing.
Zheng H and Curtis C. Cell Systems
Cornerstones of CRISPR-Cas in drug discovery and therapy.
Fellmann C, Gowen BG, Lin PC, Doudna JA, and Corn JE. Nature Reviews Drug Discovery
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Richardson CD, Ray GJ, DeWitt MA, Curie GL, and Corn JE. Nature Biotechnology
CRISPR germline engineering—the community speaks.
Bosley KS, Botchan M, Bredenoord AL, Carroll D, Charo RA, Charpentier E, Cohen R, Corn JE, Doudna JA, Feng G, Greely HT, Isasi R, Ji W, Kim JS, Lanphier E, Li J, Lovell-Badge R, Martin GS, Moreno JD, Naldini L, Pera MF, Perry ACF, Venter JC, Zhang F, and Zhou Q. Nature Biotechnology
A prudent path forward for genomic engineering and germline gene modification.
Baltimore D, Berg P, Botchan M, Carroll D, Charo RA, Church G, Corn JE, Daley GQ, Doudna JA, Fenner M, Greely HT, Jinek M, Martin GS, Penhoet E, Puck J, Sternberg SH, Weissman JS, and Yamamoto KR. Science
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
Lin S, Staahl BT, Alla RK, Doudna JA. eLife