Fall 2018
CRISPR-Cas9 basics
Paper | A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity Science | Jinek et al. | June 28, 2012 |
CRISPR-Cas9 editing in human cells
Paper | Multiplex Genome Engineering Using CRISPR/Cas Systems Science | Cong et al. | January 3, 2013 |
Additional Reading | RNA-Guided Human Genome Engineering via Cas9 – Paper Science | Mali et al. | January 3, 2013 |
CRISPR screens
Paper | Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression Cell | Qi et al. | February 28, 2013 |
Additional Reading | CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes Cell | Gilbert et al. | July 18, 2013 |
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Anti-CRISPRs
Paper | Disabling Cas9 by an anti-CRISPR DNA mimic Science Advances | Shin et al. | July 12, 2017 |
Additional Readings |
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Base editing
Paper | Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage Nature | Komor et al. | April 20, 2016 |
Additional Readings |
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Cas9 protein engineering
Paper | Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch Nature Biotechnology | Oakes et al. | May 2, 2016 |
Additional Reading | Engineered CRISPR-Cas9 nuclease with expanded targeting space – Paper Science | Nishimasu et al. | August 30, 2018 |
Discovering new CRISPR systems
Paper | Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems Molecular Cell | Shmakov et al. | October 22, 2015 |
Additional Reading | Compact CRISPR systems found in some of world's smallest microbes – General public Berkeley News | Robert Sanders | December 22, 2016 |
Nucleic acid detection with CRISPR enzymes
Paper | CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity Science | Chen et al. | February 15, 2018 |
Additional Reading | Nucleic acid detection with CRISPR-Cas13a/C2c2 – Paper Science | Gootenberg et al. | April 13, 2017 |
Delivery
Paper | Nanoparticle delivery of CRISPR into the brain rescues a mouse model of fragile X syndrome from exaggerated repetitive behaviours Nature Biomedical Engineering | Lee et al. | June 25, 2018 |
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Videos | Delivery Methods – Seminar (45 min) Dana Carroll | 2017 CRISPR Workshop |
Ethics
Paper | Gene modification therapies: views of parents of people with Down syndrome Genetics in Medicine | Michie & Allyse | June 21, 2018 |
Additional Reading | Translating dosage compensation to trisomy 21 – Paper Nature | Jiang et al. | August 15, 2018 |
Videos | Ethical and Societal Issues – Seminar (58 min) Hank Greely | 2017 CRISPR Workshop |
Treating muscular dystrophy
Paper | Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy Science | Amoasii et al. | August 30, 2018 |
Additional Readings | A CRISPR cure for Duchenne muscular dystrophy is closer after a trial in dogs – General public MIT Technology Review | Antonio Regalado | August 24, 2018 |
Domestication of crops using CRISPR
Paper | Rapid improvement of domestication traits in an orphan crop by genome editing Nature Plants | Lemmon et al. | October 1, 2018 |
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Human immune reaction to CRISPR treatments
Paper | High prevalence of Streptococcus pyogenes Cas9-reactive T cells within the adult human population Nature Medicine | Wagner et al. | October 29, 2018 |
Additional Reading | You May Already Be Immune to CRISPR – General public The Atlantic | Sarah Zhang | January 9, 2018 |