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T*
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T*
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T*
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T*
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T*
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T*
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T*
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T*
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[(ease)-842.9(Control)-841.1(and)-840.1(Prevention)-834.7(estimates)-844.5(well)-841.9(over)]TJ
0 -1.1923 TD
[(300,000)-484.1(cases)-490.4(a)-490.6(year.)-483.5(Katherine)-490(Bui,)-487.1(a)-485.2(pediatrician)-484.9(I)]TJ
T*
[(am)-305.7(somehow)-304.1(lucky)-302.9(enough)-304.7(to)-302.9(have)-300.9(married,)-307.9(says,)-303.8()51.2(The)]TJ
0 -1.1869 TD
[(West)-362.5(Coast)-354.9(has)-358.9(earthquakes.)-355.5(The)-357.5(South)-355.1(has)-358.9(hurricanes.)]TJ
0 -1.1923 TD
[(The)-276.2(middle)-272.1(of)-267.2(the)-273.2(country)-272(has)-272.2(tornadoes.)-268.5(The)-276.2(natural)-273.1(di-)]TJ
T*
[(saster)-301.7(of)-299.7(the)-300.3(Northeast)-302.7(is)-298.7(Lyme)-297.3(disease.)44.4()]TJ
1.0514 -1.1869 TD
[(The)-287.1(islands)-288.5(have)-290.1(it)-288.9(worst.)-286.1(It)-291.1(is)-287.8(an)-288.7(ecological)-291.4(problem.)]TJ
-1.0514 -1.1923 TD
[(Bluntly,)-431.7(we)-428.4(like)-431.2(to)-427.6(engineer)-430.8(the)-430.4(environment)-425.4(to)-427.6(make)]TJ
T*
[(fragmented)-460.4(forests.)-457(We)-454.1(love)-461.5(forests,)-451.5(but)-458.7(we)-455.5(cut)-457.5(them)]TJ
0 -1.1869 TD
[(up)-257.3(with)-257.8(roads)-252.1(and)-254.7(houses,)-257.6(so)-253.1(you)-255.9(get)-257(maximized)-255.4(perime-)]TJ
0 -1.1923 TD
[(ters,)-320.1(and)-314.4(we)-314.5(get)-316.6(rid)-311.3(of)-315.9(all)-318.8(the)-316.6(wolves.)-311.6(We)-318.6(maximize)-311(the)]TJ
T*
[(deer)-218.6(because)-219.7(we)-217(dont)-216.4(kill)-212.4(enough)-218(deer)-213.2(with)-219.8(automobiles,)]TJ
0 -1.1869 TD
[(let)-302.5(alone)-301.7(guns.)]TJ
1.0514 -1.1923 TD
[(A)-252.1(ton)-252.7(of)-256.3(deer)-251.1(means)-252.8(a)-257.5(ton)-252.7(of)-250.9(ticks.)-251.4(Fragmented)-257.6(forests)]TJ
-1.0514 -1.1869 TD
[(mean)-220.4(you)-218(get)-219(a)-219.6(lot)-217(more)-220.6(white-footed)-217.3(micethe)-216.7(best)-224.2(res-)]TJ
0 -1.1923 TD
[(ervoir)-326.6(of)-326.8(tick-borne)-327.5(disease.)-326.5(The)-330.4(fraction)-324.8(of)-326.8(ticks)-328(biting)]TJ
T*
[(white-footed)-439.5(mice)-439.5(relative)-435.3(to)-438.4(things)-436.2(that)-436.6(cannot)-441.3(infect)]TJ
0 -1.1869 TD
[(them)-386.5(with)-377(disease)-386.8(goes)-379.2(way)-383.6(up.)-381.3(So,)-378.1(you)-380.6(maximize)-381.5(the)]TJ
0 -1.1923 TD
[(reservoir.)-315.3(You)-314.3(maximize)-311(the)-311.2(tick)-312(vector.)-314.9(You)-308.9(maximize)]TJ
T*
[(the)-300.3(number)-299.1(of)-299.7(infected)-303(ticks.)]TJ
/F4 1 Tf
/Cs8 cs 1 scn
0 -2.428 TD
(Davies)Tj
/F5 1 Tf
2.7803 0 TD
[(:)-284.8(What)-281.4(is)-282.4(the)-283(strategy)-283.7(to)-278(combat)-281.8(this)-286.2(problem?)]TJ
0 0 0 rg
-2.7803 -2.428 TD
(Esvelt:)Tj
/F6 1 Tf
3.1651 0 TD
[(Tackle)-217.7(the)-219(mice.)-216.5(Can)-218.8(we)-217(use)-218(CRISPR)-217.8(to)-221.6(engineer)]TJ
-3.1651 -1.1923 TD
[(immunity)-327.7(into)-324(the)-322(mice?)-328.5(Because)-324.6(these)-330(are)-323(islands,)-325.7(you)]TJ
0 -1.1869 TD
[(have)-366(two)-359.9(optionsnormally)-358.8(you)-358.9(would)-362.5(only)-359.7(have)-360.5(one.)]TJ
0 -1.1923 TD
[(If)-564.2(you)-554(release)-562.3(enough)-559.5(mice)-558.7(with)-561.3(engineered)-556.9(genetic)]TJ
/F11 1 Tf
9.9626 0 0 9.9626 62.022 754.3557 Tm
(322)Tj
34.9001 0 TD
[(INTERV)-8.3(IEW)-300.4(WITH)-309(KEVIN)-306.4(ESVE)-8.4(LT)]TJ
ET
endstream
endobj
44 0 obj
<>stream
/GS1 gs
BT
/F6 1 Tf
10.4607 0 0 10.4607 59.7543 698.2297 Tm
0 0 0 rg
0 Tc
0 Tw
[(cassettes)-223.7(and)-216.8(provide)-217.8(resistance)-220.1(to)-221.6(either)-220.3(Lyme)-216(disease)-218.8(or)]TJ
0 -1.1923 TD
[(to)-292.1(tick)-284.9(bites)-290.1(directly,)-289.4(then)-288.1(you)-283(can)-291.5(disrupt)-287.5(the)-289.5(ecological)]TJ
T*
[(cycle)-246.4(of)-245.5(transmission.)-240.6(That)-244.5(is,)-243.8(if)-247.7(you)-239.7(make)-247.5(this)-243.9(immunity)]TJ
0 -1.1869 TD
[(heritable)-227.9(and)-227.7(release)-226.3(enough)-228.9(mice)-222.7(with)-230.7(protective)-226.5(alleles,)]TJ
0 -1.1923 TD
[(on)-295.3(average,)-298(descendants)-298.7(will)-297.9(inherit)-292.7(at)-296.3(least)-299.7(one)-298.1(copy)-291.3(of)]TJ
T*
[(protective)-302.4(alleles)-298(and)-303.5(disrupt)-298.3(the)-300.3(cycle.)]TJ
1.0514 -1.1869 TD
[(There)-265.8(are)-268.8(only)-267.6(so)-269.4(many)-265(mice)-271.4(there)-268.2(on)-268.2(the)-267.8(islands,)-266.1(and)]TJ
-1.0514 -1.1923 TD
[(there)-219.4(is)-212(not)-214.8(a)-214.2(whole)-215(lot)-211.6(of)-218.4(gene)-214.2(ow)-213.6(with)-214.4(the)-213.6(mainland,)-213.2(so)]TJ
T*
[(you)-489(just)-487.8(need)-490.6(to)-487.2(release)-486.4(enough)-489(heritably)-489.3(immunized)]TJ
0 -1.1869 TD
[(mice.)-314.1(As)-306.1(long)-311(as)-311.5(youre)-308.1(not)-306.9(using)-310.7(a)-311.7(gene)-306.3(drive,)-312.1(it)-310.6(is)-309.5(not)]TJ
0 -1.1923 TD
[(going)-407.1(to)-400.5(spread)-406.7(on)-403.7(the)-408.7(mainland.)-402.8(So,)-405.2(it)-402.7(should)-401.5(be)-407.9(just)]TJ
T*
[(an)-299.5(island-by-island)-298.8(thing.)]TJ
1.0514 -1.1869 TD
-.0158 Tc
[(I)-371.1(t)-.4(h)-3.6(oug)-3.6(ht)-374.3(we)-370.9(mig)-3.6(h)1.9(t)-374.3(b)1.9(e)-376.3(a)-2.4(ble)-370.9(t)-15.8(o)-362.1(d)1.9(o)-372.1(t)-.4(h)-3.6(i)-.4(s)-373.3(b)1.9(ecau)-3.6(s).7(e)-370.9(w)-2.3(e)-376.3(a)3.1(re)]TJ
-1.0514 -1.1923 TD
-.0146 Tc
[(pr)4.1(etty)-295(good)-295(at)-291.8(engineering)-295(r)4.1(od)]TJ
11.9232 0 TD
-.0144 Tc
[(ents.)-290.1(W).4(hite-footed)-289.4(m)-3(ice)-293.6(a)4.5(re)]TJ
-11.9232 -1.1923 TD
0 Tc
[(actually)-289.9(more)-291.1(distant)-284.1(from)-291.3(lab)-284.1(mice)-293.1(than)-288.1(lab)-289.5(mice)-287.7(are)-290.5(to)]TJ
0 -1.1869 TD
.0198 Tc
[(r)-4.9(a).7(ts,)-185.4(but)-187(they)-184.7(a)-4.7(r).5(e)-189(s)3.7(till)-187(r).5(odents.)-190.9(There)-189(i)2.7(s)-191.4(a)-189(vaccine)-189(against)]TJ
0 -1.1923 TD
.0202 Tc
[(Lyme)-329.5(disease.)-326(We)-329.5(know)-329.5(it)-332.9(is)-331.9(antibody)-330.7(med)5.3(i)-2.3(ated.)-331.4(I).9(t)-327.5(i)-2.3(s)]TJ
T*
0 Tc
[(possible)-335.8(that)-339.1(tick)-339.1(resistance)-339.3(is)-336.6(antibody)-333.5(mediated.)-336.6(If)-336.6(so,)]TJ
0 -1.1869 TD
[(in)-357.1(either)-355.7(case,)-359.8(we)-357.9(can)-356.5(isolate)-358.8(antibodies)-354.6(that)-355.3(are)-355.5(highly)]TJ
0 -1.1923 TD
(protective.)Tj
1.0514 -1.1923 TD
[(If)-287.8(we)-287.4(were)-293.3(to)-286.7(encode)-286.1(those)-293.3(antibodies)-284.1(in)-286.7(the)-289.5(germline)]TJ
-1.0514 -1.1869 TD
[(such)-260(that)-263.2(newborn)-257.8(mice)-255.2(begin)-259.6(expressing)-258.2(them,)-261.1(then)-255.6(you)]TJ
0 -1.1923 TD
[(could)-265(make)-258.4(mice)-266(that)-257.8(would)-259.6(be)-267(heritably)-256.2(resistant)-264(to)-259.6(dis-)]TJ
T*
[(ease,)-327.3(take)-319.4(that)-322.8(acquired)-322.4(resistance)-323(from)-318.4(some)-320.4(mice,)-324.9(and)]TJ
0 -1.1869 TD
[(put)-301.5(it)-299.7(back)-300.9(into)-296.9(mice.)]TJ
1.0514 -1.1923 TD
[(I)-398.6(w)13.4(as)-392.8(w)13.4(e)0(l)18(l)-396.5(aw)16(a)13.4(r)0(e)-390.6(o)0(f)-391.8(p)12.2(eo)20.3(p)12.2(l)0(e)18()13.2(s)-400.8(w)0(a)21.5(r)0(i)17.8(n)12.2(es)19.1(s)-400.8(o)12.2(f)-398.6(g)0(e)20.3(n)0(e)20.3(t)0(i)20(c)0(a)16(l)0(l)20(y)]TJ
-1.0514 -1.1869 TD
[(mo)18.2(d)12.2(i)0()20.1(e)0(d)-169.4(o)12.2(rg)20.1(an)20.3(is)21.1(ms)-172.6(\()13.2(G)0(M)20.5(O)0(s)19.1(\))-176.4(bu)19.1(t)-179.7(s)11(p)12.2(e)0(c)16(i)0()20.1(c)13.4(al)18(ly)-167.4(o)12.2(f)-181.9(G)13.5(M)0(O)20.5(s)-178.6(cr)15.8(e)13.4(-)]TJ
0 -1.1923 TD
-.0123 Tc
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T*
0 Tc
[(vi)16.8(e)13.4(w)-197.9(th)16.8(is)-184.9(as)-186.9(un)19.1(n)12.2(a)0(t)18(u)12.2(ra)15.8(l.)-184.4(Wh)16.2(e)13.4(n)-199.1(we)-189.9(a)13.4(p)0(p)19.1(r)0(o)20.1(a)0(c)21.4(h)0(e)20.3(d)-199.1(co)20.3(m)11.4(m)11.4(u)0(n)19.1(i)0(t)20(i)0(e)18(s)11(,)]TJ
0 -1.1869 TD
[(on)-176.1(th)22.3(e)-181.7(a)0(d)20.3(v)12.2(ic)18(e)-181.7(o)0(f)-175(t)0(h)22.3(e)-187.1(lo)22.3(ca)21.5(l)-185.1(r)13.2(es)19.1(id)16.8(e)13.4(n)0(t)16.8(s)11(,)-183.6(t)0(h)22.3(e)0(y)-174.9(j)0(u)22.3(s)11(t)-185.1(s)11(a)13.4(i)0(d)16.8(,)-183.6()62()0(C)18.1(o)12.2(nt)16.8(a)13.4(c)0(t)]TJ
0 -1.1923 TD
[(th)16.8(e)-300.9(B)10.2(o)12.2(a)0(r)21.3(d)0(s)-291.1(o)12.2(f)-301.1(H)0(e)16.1(a)13.4(lt)20(h,)-290.6(h)12.2(a)0(v)20.3(e)-300.9(a)-300.9(t)0(o)16.8(w)13.4(n)-302.1(h)12.2(al)18(l)-298.9(m)11.4(ee)16(ti)20(n)12.2(g)12.2(.)0()62.7()-295.7(Th)19.2(is)-293.3(is)]TJ
T*
[(Ne)16.1(w)-241.3(E)12.4(ng)19.1(la)18(n)12.2(d)-242.5(to)22.3(wn)-234.4(h)12.2(a)0(l)18(l)-239.3(d)12.2(e)0(m)19.4(o)0(c)20.3(r)0(a)15.8(c)13.4(y.)-230.9(Th)19.2(e)-241.3(B)10.2(oa)20.3(rd)20.1(s)-243.7(o)12.2(f)-246.9(H)13.4(ea)16(lt)20(h)]TJ
0 -1.1869 TD
[(wi)18.1(ll)-261.8(ju)22.3(s)11(t)-271.8(a)13.4(d)0(v)19.1(e)0(r)21.3(t)0(i)20(s)0(e)-262.8(t)0(h)22.3(e)-268.4(me)19.4(et)18(in)22.3(g.)-258(Th)19.2(ey)-261.6(wi)18.1(l)15.4(l)-271.8(g)12.2(e)0(t)-263.8(a)-268.4(fe)21.3(w)-273.8(d)12.2(o)12.2(z)0(e)16(n)]TJ
0 -1.1923 TD
[(lo)16.8(c)13.4(a)0(l)-242.1(r)0(e)21.3(s)0(i)15.7(d)12.2(en)20.3(ts)-239.1(co)20.3(m)11.4(e)-252.1(in)-237.9(to)-237.9(he)20.3(ar)-244.3(y)12.2(o)12.2(u)-253.3(ta)23.5(lk)16.8(,)-248.6(a)13.4(nd)-241.1(th)22.3(ey)-239.9(wi)18.1(ll)-240.1(y)12.2(e)0(l)18(l)]TJ
T*
[(at)-274.6(yo)19.1(u)-285.8(a)13.4(nd)-273.6(te)18(ll)-272.6(y)12.2(o)0(u)-273.6(i)0(f)-274.8(t)0(h)16.8(e)13.4(y)-285.8(a)0(r)21.3(e)-284.6(in)22.3(te)18(re)15.8(s)11(t)0(e)23.5(d)0()20.5(w)0(h)20.3(i)0(c)18(h)-280.4(is)-277(p)12.2(r)0(e)15.8(t)0(t)20(y)]TJ
0 -1.1869 TD
[(mu)18.2(ch)-229.1(h)12.2(o)0(w)-229(i)0(t)-234.7(h)12.2(ap)20.3(p)12.2(e)0(n)20.3(e)0(d)20.3(.)]TJ
1.0514 -1.1923 TD
[(As)-333.2(I)-339(recall,)-332.2(the)-338.3(communities)-333.1(said,)-337.3()51.2(Yes,)-338.3(we)-336.2(are)-333.8(very)]TJ
-1.0514 -1.1923 TD
[(interested)-460.7(in)-460.1(this,)-454.6(but)-458.7(we)-455.5(would)-460.1(really)-458.7(rather)-455.5(you)-456.5(not)]TJ
0 -1.1869 TD
[(use)-543.2(any)-542(foreign)-542(DNA.)-538.8(If)-542.6(you)-537.8(are)-545.2(going)-537.2(to)-541.4(engineer)]TJ
0 -1.1923 TD
[(mice,)-427.9(we)-428.4(would)-427.6(prefer)-425.2(them)-429.8(to)-422.2(be)-429.6(100%)-425.6(mouse,)-426.7(as)-425.3(it)]TJ
T*
[(were.)-596.1(But)-590.8(please)-593(immunize)-588.6(them)-592.4(against)-595(everything)]TJ
0 -1.1869 TD
[(you)-380.6(can.)47.6()-382.4(This)-382.4(notion)-380.8(of)-381(using)-375.8(antibodies)-381.7(is)-380(great,)-381.3(but)]TJ
0 -1.1923 TD
[(if)-377.8(you)-375.2(are)-377.2(not)-377.4(using)-370.4(any)-379.4(foreign)-374(DNA,)-376.2(that)-377(means)-372(you)]TJ
T*
[(cannot)-300.3(do)-300.7(any)-298.1(form)-296.7(of)-299.7(gene)-300.9(drive,)-301.2(even)-300.9(a)-295.5(local)-304(drive.)]TJ
/F4 1 Tf
/Cs8 cs 1 scn
0 -2.3304 TD
(Davies)Tj
/F5 1 Tf
2.7803 0 TD
[(:)-680.5(Has)-675.3(the)-678.7(local)-679.1(community)-675.3(given)-679.1(you)-675.4(the)]TJ
-2.7803 -1.1923 TD
[(thumbs-up)-298.6(to)-299.7(go)-300.7(ahead?)]TJ
0 0 0 rg
0 -2.428 TD
(Esvelt:)Tj
/F6 1 Tf
3.2789 0 TD
[(They)-334.5(have)-333.4(established)-336.9(steering)-332.5(committees,)-332.6(one)]TJ
-3.2789 -1.1923 TD
[(for)-487(each)-489.4(island,)-483.1(and)-482.4(the)-490(steering)-484.2(committees)-485.1(can)-486.6(end)]TJ
0 -1.1869 TD
[(the)-327.4(project)-327.2(for)-324.4(their)-331.2(island)-326.6(at)-323.4(any)-325.2(time,)-328.4(just)-325.2(by)-327.8(calling)-325.1(a)]TJ
0 -1.1923 TD
[(vote)-353.1(and)-346.9(saying)-351.5(no,)-348.8(and)-352.3(that)-349.9(is)-347.5(it.)-353.2(Then)-345.3(we)-352.5(walk)-351.9(away.)]TJ
24.0035 60.7159 TD
[(They)-247.7(tend)-244.7(to)-248.7(be)-245.3(packed)-248.1(with)-246.9(extremely)-248.2(well-credentialed)]TJ
0 -1.1923 TD
[(people)-240.7(because)-246.7(that)-241.5(is)-244.5(the)-246.1(nature)-245.1(of)-240.1(the)-246.1(islands.)-244.4(The)-243.7(chair)]TJ
T*
[(of)-418.9(the)-425(Nantucket)-420.6(steering)-424.6(committee,)-419.5(Howard)-420.5(Dickler,)]TJ
0 -1.1869 TD
[(was)-411.9(in)-411.3(charge)-415.2(of)-408.1(the)-414.1(National)-409.1(Institute)-412.6(of)-413.5(Allergy)-408.5(and)]TJ
0 -1.1923 TD
[(Infection)-339.7(Diseases)-339.6(immunology)-338.9(branch.)-334.4(John)-342.4(Goldman)]TJ
T*
[(is)-331.2(an)-326.6(editor)-329.8(of)-332.2(the)]TJ
/F7 1 Tf
7.6633 0 TD
[(Journal)-328.4(of)-330(Immunology)]TJ
/F6 1 Tf
9.5494 0 TD
[(,)-329.9(and)-330.6(they)-331.4(are)]TJ
-17.2127 -1.1869 TD
[(two)-300.3(on)-295.3(the)-300.3(Nantucket)-301.4(steering)-299.9(community.)]TJ
1.046 -1.1923 TD
[(Also)-294.7(on)-295.3(the)-294.9(committee)-290.2(is)-298.7(Danica)-292.5(Connors,)-295.2(who)-292.7(is)-293.3(ab-)]TJ
-1.046 -1.1923 TD
[(solutely)-239.3(wonderful.)-239.2(She)-240.7(is)-239.1(a)-246.7(herbalist,)-240.4(deeply)-240.7(skeptical)-241(of)]TJ
0 -1.1869 TD
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0 -1.1923 TD
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T*
[(done)-215.4(because)-214.2(if)-220.6(everything)-214.7(turns)-212.2(out)-214.8(perfectly,)-218.6(maybe)-219.1(she)]TJ
0 -1.1869 TD
[(could)-297.5(support)-296.1(it.)]TJ
1.046 -1.1923 TD
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-1.046 -1.1923 TD
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