The heart of this research program is to overturn the existing one-gene-at-a-time paradigm for probing gene function in model organisms, and push the envelope for understanding genetic interaction in vivo. We will genetically modify  model organisms using a high-throughput CRISPR-based platform suitable for interrogating pathways. This platform will allow the interrogation of thousands of targeted gene combinations in parallel in whole organisms. A high-throughput, targeted, multi-mutation approach has never been accomplished in any model organism. If successful, our strategy will revolutionize the development of genetically engineered complex model organisms.