Traditional techniques in experimental cell biology require either direct observation or destructive analysis of the cells being studied. This is problematic when studying a complex or developing systems that change over time. To circumvent this limitation we are taking a new approach: the logging of biological events as coded nucleotides, written into the genome of a living cell. These organized changes to genomic DNA enable us to store data during an event of interest, without interrupting the ongoing biological processes, to be collected later by sequencing. This new type of molecular data collection will allow us to probe the complex and developing systems in a different dimension and build out a more complete framework cell biology that occurs over time.
Molecular Recording: Capture and Storage of Data Within the
Genome of a Cell Using CRISPR Integrases
Assistant Investigator, Gladstone Institute of Data Science and Biotechnology
Assistant Professor, Department of Bioengineering & Therapeutic Sciences
University of California, San Francisco