The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. Dr. Komor will describe the development of base editing, a new approach to genome editing that enables the direct, irreversible conversion of one target DNA base into another in a programmable manner, without requiring double-stranded DNA backbone cleavage or a donor template. Using protein engineering and directed evolution approaches, she has re-purposed two types of nucleobase deaminase enzymes into genome editing agents that are capable of facilitating all four types of transition mutations (C to T, G to A, T to C, and A to G) in the genome of living cells.
Performing Chemistry on the Genome
Assistant Professor, Department of Chemistry and Biochemistry
University of California, San Diego