
This 1-week workshop focused on applications of CRISPR technology as a platform for genome editing and functional genomics. The program included lectures from experts in the field and a hands-on laboratory experience demonstrating targeted mutagenesis in cultured human cells. On this page, you can browse recorded videos of the faculty lectures and read through the lab syllabus, which includes easy-to-follow protocols.
Follow these links to view videos from the 2015 and 2016 CRISPR Workshops
Day 1:
Protocol: In vitro T7 template synthesis and transcription
Day 2:
Protocol: RNP Nucleofection of HEK293T Cells
Day 3:
Day 4:
Protocol: Genomic DNA Extraction, Amplification, and T7E1 Assay
Day 5: