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2017 CRISPR Workshop: Practical Aspects of Precision Biology

This 1-week workshop focused on applications of CRISPR technology as a platform for genome editing and functional genomics. The program included lectures from experts in the field and a hands-on laboratory experience demonstrating targeted mutagenesis in cultured human cells. On this page, you can browse recorded videos of the faculty lectures and read through the lab syllabus, which includes easy-to-follow protocols.

Follow these links to view videos from the 2015 and 2016 CRISPR Workshops

Video Presentations

Dana Carroll Speaking at CRISPR Workshop 2017
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Background on Genome Editing

Dana Carroll

Professor, University of Utah
Jennifer Doudna speaking at CRISPR Workshop 2017
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CRISPR Basics

Jennifer Doudna

Executive Director, IGI; Professor and Howard Hughes Investigator, UC Berkeley
Dana Carroll speaking at CRISPR Workshop 2017
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Issues in CRISPR-Cas Editing

Dana Carroll

Professor, University of Utah
Luke Gilbert speaking at CRISPR Workshop 2017
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Introduction to dCas9

Luke Gilbert

Assistant Professor, UCSF
Luke Gilbert speaking at CRISPR Workshop 2017
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Repurposing CRISPR as an RNA-guided DNA binding platform

Luke Gilbert

Assistant Professor, UCSF
Mike Bassik speaking at CRISPR Workshop 2017
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Multiplexing with CRISPR Screens

Mike Bassik

Assistant Professor of Genetics, Stanford
Jennifer Listgarten speaking at CRISPR Workshop 2017
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CRISPR Bioinformatics: Machine learning predictive models for guide design

Jennifer Listgarten

Microsoft, Inc.
Dana Carroll speaking at CRISPR workshop
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Delivery Methods

Dana Carroll

Professor, University of Utah
Joanna Yeh speaking at CRISPR workshop
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CRISPR Applications: Zebrafish

Joanna Yeh

Assistant Professor, Harvard Medical School
Lin He speaking at CRISPR Workshop 2017
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CRISPR Applications: Mouse

Lin He

Associate Professor of Cell and Developmental Biology, UC Berkeley
Ethan Bier speaking at CRISPR Workshop 2017
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CRISPR Applications: Gene Drives

Ethan Bier

Professor, UCSD
Don Kohn speaking at CRISPR Workshop 2017
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CRISPR Applications: Gene Editing in Hematopoietic Stem Cells

Don Kohn

Professor, UCLA
Hank Greely speaking at CRISPR Workshop 2017
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Ethical & Societal Issues

Hank Greely

Professor of Law and Director of the Center for Law and the Biosciences, Stanford Law
Richard Hynes speaking at CRISPR Workshop 2017
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NAS Report

Richard Hynes

Professor and Howard Hughes Investigator, MIT

Lab Syllabus

Day 1:

  • Template PCR
  • Agarose gel on PCR products
  • Set up overnight transcription

Protocol: In vitro T7 template synthesis and transcription

Day 2:

  • Purify sgRNAs
  • Determine sgRNA concentration
  • Run RNA gel
  • Assemble Cas9 RNPs
  • Nucleofect HEK293T cells

Protocol: RNP Nucleofection of HEK293T Cells

Day 3:

  • Monitor cells
  • Bioinformatics session

Day 4:

  • Harvest cells
  • Prepare genomic DNA (Quick Extract)
  • PCR amplify targets
  • Run agarose gel

Protocol: Genomic DNA Extraction, Amplification, and T7E1 Assay

Day 5:

  • T7E1 assay
  • Run agarose gel
  • Assess results

Sponsors

Logos of sponsors for the CRISPR Workshop